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KMID : 0380619860180020088
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Korean Journal of Food Science and Technology
1986 Volume.18 No. 2 p.88 ~ p.92
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Determination of Intracellular ATP of bacteria on the surface of Chicken
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Abstract
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Determination of bacterial adenosine triphosphate (ATP) based on luciferin-luciferase bioluminescene reaction was applied to the measurement of bacteria on the surface of chicken as an alternative rapid method. The light yield was proportional to the concentration of ATP giving a straight line within a range of 10^(-10) to 10^(_6)M. The bacteria isolated from the surface of chicken were identified as Escherichia coli, Hafnia alvei, Pseudomoszas putida and Aeromoncts hydsophila. When the ATP contents of each bacteria were determined by bioluminescence reaction and compared, there was no significant difference (r=0.95). The patterns of growth curves for E. coli look very similar, when the bacterial growth was monitored by ATP content and viable cell count. When bacterial ATP of each samples collected every 2 days during storage for 14 days at 4℃ was determined and compared with viable cell count, it gave a good correlation (r=0.95, n=32).
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